The show begins with a countdown displayed on a large screen, with the line "Don't call me Gaga" repeatedly played from the speakers. After Gaga shouts out that she instead wants to be called Joanne, she starts performing "Diamond Heart”, standing solely on a raised platform with a microphone stand. She then moves on to sing "A-Yo", while playing on a guitar, and her dancers and guitarists also appear on stage. The show then continues with a choreographed performance of "Poker Face" with her dancers clad in black cowboy-style leather costumes. The first act of the show is concluded by "Perfect Illusion", followed by a video interlude which shows Gaga driving a vintage convertible letting out pink smoke. "John Wayne" is performed with pyrotechnic effects alongside her dancers. This is followed by "Scheiße", performed on the raised platforms. nAfter a solo performance of "Alejandro", Gaga leaves the stage for a costume change.
Following another video interlude, depicting the singer with rhino horns, Gaga appears onstage playing her keytar to "Just Dance". "LoveGame" and "Telephone" follows the performance, the former using her characteristic disco-stick. During "Applause", the three lightning pods that were used for video projection are lowered, forming bridges for Gaga and her dancers to cross between the b-stages, allowing them to get to the piano by the end of the song. Here Gaga gives a speech to the crowd and then starts singing "Come to Mama" and "The Edge of Glory" on the piano. Changing into a ball gown Gaga performs "Born This Way" accompanied by colorful lighting effects.
After another video interlude, Gaga returns to sing "Bloody Mary" in a puffer coat red dress, which is changed into a red bodysuit for "Dancin' in Circles". Gaga then performs "Paparazzi" portraying a choreographed battle onstage, showing someone stalked and murdered. Ambulance sirens are heard and a cross with a red and blue background is displayed as one of the pods are lifted, containing her. The show then shifts into "Angel Down" as the pod that contained Gaga, now in a white fringe blazer and a brownish hat with a feather, is lowered halfway. Gaga then goes down to the platform to tell the audience a story about her late aunt, Joanne Germanotta, before sitting down on a stool, guitar in-hand to perform the album title track.​
The final segment begins with "Bad Romance", with Gaga and her dancers all decked in white, followed by "The Cure" with full choreography. She takes a bow and leaves the stage, only to return a few minutes later for the encore, wearing a long silver sparkling jacket paired with the signature pink hat. "Million Reasons" is sung on piano, with the singer standing atop it, and the show ends with Gaga disappearing under the stage, while leaving the pink hat behind on the piano stool.
